Fig. 2.
Oligomeric structures of dynamins are visualized using negative stain TEM. (A) Oligomers are generated under low salt conditions or in the presence of nonhydrolyzable nucleotides. (B) Dynamin spiral structures are shown that were dialyzed in the presence of GDP/BeF. (C) Larger spiral structures are observed for Dnm1 in the presence of GMP-PCP. (D) MxA curved filaments and occasional rings are presented after incubation with GMP-PCP. All protein structures were generated in HCB100. Scale bar, 100 nm.