Fig. 4.

Biochemical assays quantify dynamin assembly and conformational changes upon addition of GTP. (A) The sup/pellet assay quantifies dynamin assembly under different conditions by centrifuging the sample at 100,000g and running the supernatant and pellet fractions on a gel (top). Imaging software quantifies the relative amount of protein in the pellet (bottom). (B) 90° light scattering measures the relative change in tube conformation. Addition of GTP (indicated with an arrow) leads to a rapid and dramatic decrease in scattering.