A. ChIP was performed on ML-2 cells using anti-MLLN, anti-menin and anti-LEDGF antibodies. MLL-AF6, menin and LEDGF occupancy was specifically observed at the HOXA7 and HOXA9 loci, but not on the MEIS1 and GAPDH loci. Negative and positive controls consisted of no antibody and anti-histone H3 antibody, respectively.
B. ChIP followed by quantitative PCR was performed on ML-2 and U937 cells using anti-MLLN, anti-menin and anti-LEDGF antibodies. Values are expressed relative to the maximum value (arbitrarily set at 100%) in each group with error bars representing standard deviations for triplicate PCR analyses.