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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Nat Genet. 2004 Nov 14;36(12):1331–1339. doi: 10.1038/ng1473

Figure 4. EMSAs of PBM-derived Rap1 binding site sequences.

Figure 4

Figure 4

(a) Rap1 binding site sequences present within the DNA probes corresponding to portions of the intergenic regions iYLL051C (P = 3.20 × 10-16) and iYPL221W (P = 3.91 x 10-21), aligned against the TRANSFAC and PBM-derived Rap1 binding site sequence logos. (b) Lanes 1 and 2, positive control DNA probe; lanes 3 and 4, negative control DNA probe; lanes 5 and 6, DNA probe corresponding to the best Rap1 binding site sequence that could be identified within the iYLL051C intergenic region; lanes 7 and 8, DNA probe corresponding to the PBM-derived Rap1 binding site sequence within the iYPL221W intergenic region. “-” indicates Rap1 protein was not present in the binding reaction; “+” indicates Rap1 protein was present the binding reaction.