Table 2.
Frataxin Lateral Flow Assay Intra- and Inter-assay Imprecision.
| Intra-assay Imprecision | ||||||
|---|---|---|---|---|---|---|
| Experiment | Dipstick 1 | Dipstick 2 | Dipstick 3 | Mean | SD | CV (%) |
| Day 1 | 19,380 | 17,696 | 19,168 | 18,748 | 917 | 4.9 |
| Day 2 | 19,867 | 19,535 | 18,260 | 19,221 | 848 | 4.4 |
| Day 3 | 19,107 | 18,531 | 18,343 | 18,660 | 398 | 2.1 |
| Day 4 | 21,502 | 23,164 | 23,717 | 22,794 | 1,152 | 5.1 |
| Inter-assay Imprecision (Day 1- Day 4) | ||||||
|---|---|---|---|---|---|---|
| Mean Signal | SD | CV (%) | ||||
| 19,856 | 1,974 | 9.9 | ||||
A single sample of normal cultured fibroblast whole cell extract (within working range of assay) was prepared as described in the Materials and Methods. Total protein concentration of the extract was determined by the BCA method, aliquots frozen at −80° C and then retrieved each day for analysis. Dipstick signal was determined using a Hamamatsu ICA immune-chormato reader and is expressed as Area units. Intra-assay imprecision was determined from n=3 dipsticks/ day. Inter-assay imprecision data was compiled from 4 separate days. Coefficient of variation (CV) was determined by the ratio of standard deviation (SD)/Mean × 100.