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. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: Anal Biochem. 2009 Apr 5;389(2):177–179. doi: 10.1016/j.ab.2009.03.044

Figure 2. Generation of highly pure ssDNA.

Figure 2

(A) Electrophoretic analysis of the ssDNA products. Lane1 is ssDNA isolated with silica column from the culture of JM109/pGEM5Z(+)-EGFP/M13KO7 complex. Lane2 is ssDNA isolated from M13KO7-infected JM109/pGEM5Z(+)-EGFP culture with the conventional method. Lanes 3 and 4 are the same ssDNA samples as Lanes 1 and 2, respectively, after treatment with DSN. (B) Fluorescent images of HCT116 cells co-transfected with ssDNA and pDsRed1-N1. ssDNA (3 μg) isolated from JM109/pGEM5Z(+)-EGFP/M13KO7 complex was treated with or without DSN, and then co-transfected with pDsRed1-N1 (1 μg) into HCT116 cells with Lipofectamine 2000 (Invitrogen, Carlsbad, CA). The representative green and red fluorescent images were taken 24 hr later. Co-transfection with pDsRed1-N1 was used to ascertain that the transfection efficiencies were comparable.