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Human islets (n=5) incubated in ABO-compatible hirudin-treated plasma (n=5) for 30 min. The islets were stained for IgG, IgM, MBL, C1q, C4, C3b/iC3b, C9, and negative control. Controls consisting of islets incubated without (islets alone) and with mouse isotype IgG1 (negative control) were included. The islets were analyzed by (A) COPAS and (B) confocal microscopy. All values were compared with values obtained from the negative control are given as mean±SEM (*P<0.05, **P<0.01).
