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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Osteoarthritis Cartilage. 2008 Sep 30;17(4):513–517. doi: 10.1016/j.joca.2008.08.003

Table 1.

Primer sequences for Real-time PCR

Gene Primer sequences(forward/reverse)(5′-3′) Size(bp) Annealing temperature(°C) Reference Accession no.
GAPDH TCGACAGTCAGCCGCATCTTCTTT
GCCCAATACGACCAAATCCGTTGA		 148 55 EMBL:NM_002046
Aggrecan TCTTGGAGAAGGGAGTCCAACTCT
ACAGCTGCAGTGATGACCCTCAGA		 89 55 EMBL:NM_013227
IGF-1 TGGTCCTGGAGTTGGTAGATTGCT
CACCCATGCATTTGTGGCTCTTGA		 124 55 NM_000618.2
IGF-1R TGATCCTGGATGCGGTGTCCAATA
TGGTCTTCTCACACATCGGCTTCT		 111 55 NM_000875.3
OP-1 AGGGCTGGCTGGTGTTTGACAT
AGAGCTGCAGGCCCAGGTTGT		 81 58 NM_001719.1