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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Mol Microbiol. 2009 Jan 16;71(6):1386–1401. doi: 10.1111/j.1365-2958.2009.06610.x

FIG. 6. Inhibition of L. major DHCH1 by 5,10-CO-THF.

FIG. 6

The dehydrogenase activity of purified recombinant DHCH1 (1.3 μg was assayed in the presence of 1 mM NADP+ and varying concentrations of 5,10-CH2-THF substrate (12.5 to 250 μM) in the presence of no (●), 1.5 μM (○), 3 μM (▼) or 5 μM (▽) inhibitor. The lines show a global fit of these data to a simple competitive mode of inhibition.