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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Mol Biochem Parasitol. 2009 Apr 5;166(2):142–152. doi: 10.1016/j.molbiopara.2009.03.009

Figure 6. Immunolocalization of L. infantum DHCH2.

Figure 6

L. donovani transfected with pXG-LiDHCH2-GFP were stained with an anti-GFP antibody (green, panel A), Mitotracker Red CMXRos (red, panel B) and Hoechest 33342 (blue, panel C). The kinetoplast (K) and nucleus (N) labeled with arrows. Merged fluorescence images are shown in panel D and the corresponding differential interference contrast image is shown in panel E, scale bar is 2 μm. No GFP staining was seen in negative controls where the primary anti-GFP antibody was omitted, or in untransfected wild-type cells (not shown).