Table 2.
Kinetic constants of Leishmania major FTL
| Substrate | Km (app) (μM) | kcatd (s−1) | kcat/Km(M−1 s−1) × 104 |
|---|---|---|---|
| THF a | > 1,500 | - | - |
| ATP b | 360 ± 50 | 3.7 ± 0.1 | 1 |
| Formate c | 21 ± 2 | 4.4 ± 0.1 | 21 |
Data are from a single determination and are given with the standard error of a non-linear fit to the Michaelis-Menten equation.
a
Measured with 5 mM ATP and 200 mM formate as the fixed substrates.
b
Measured with 1 mM THF and 200 mM formate as the fixed substrates.
c
Measured with 1 mM THF and 5 mM ATP as the fixed substrates.
d
Calculated assuming one active site per polypeptide.