Fig. 2.

(Left) A 2bp CPB produces a strong, three-fold enhancement in fluorescence upon target binding. (Right) In contrast, a three base pair construct exhibits only a small (~15%) increase in signal and a PB construct lacking PNA base pairs does not produce any observable gain. Here the intensity of the construct in the absence of antibody is represented by a dashed line; the intensity of the CPBs in the presence of saturating (40–80 nM) antibody is represented by a solid line.