Figure 1. Dose titration of Ad5 [E1-]-gag and Ad5 [E1-, E2b-]-gag and resultant CMI responses.
Naïve BALB/C mice (n=5/group) were immunized three times using doses of 107, 108, 109 or 1010 VP of Ad5 [E1-, E2b-]-gag. Fourteen days after the final immunization splenocytes from the mice were assessed by ELISpot analysis. The greatest induction of CMI was achieved by using 1010 VP of the vector. White = Ad5 [E1-]-gag; Black = Ad5 [E1-, E2b-]gag. The error bars depict the standard error of the mean (SEM).