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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Cell Signal. 2009 Mar 16;21(7):1169–1179. doi: 10.1016/j.cellsig.2009.03.006

Figure 9. Effect of overexpressing p25 on cellular Arf1•GTP levels.

Figure 9

Cos7 cells were transfected with plasmids directing the expression of Arf1-HA and myc-p25 as indicated. 24 hours after transfection cells were lysed and cellular levels of Arf1•GTP were determined by selective precipitation of Arf1•GTP with GST-GGA as described in “Materials and Methods.” The primary data from one experiment is shown in panel A and a summary of three experiments in which signal was quantified as described in “Materials and Methods” is shown in panel B. **, different than control, p<0.01 by Student t-test.