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. Author manuscript; available in PMC: 2009 Jun 8.

Published in final edited form as: Exp Cell Res. 2008 Jan 16;314(6):1237–1249. doi: 10.1016/j.yexcr.2007.12.028

Fig. 5 — Physiological concentration of K⁺ blocks Ca²⁺-dependent DNA degradation in nuclei. (a) TUNEL assay, Nuclei (1 × 10⁴) were incubated under “apoptotic” (40 mM) and “physiological” concentration (140 mM) of K⁺ with 1 mM CaCl₂. Right, fluorescent TUNEL assay. Left, counter staining with DAP I. The data shown represents at least three experiments. (b) Analysis of Ca²⁺-dependent DNA degradation under various concentration of K⁺. DNA was extracted and run on agarose gel electrophoresis. Nuclei (2 × 10⁶) were incubated with 1 mM CaCl₂ together with an increasing concentration of KCl. For each sample, 1 μg of DNA was analyzed. “Ø” denotes control nuclei maintained on ice. The data shown represents one of three independent experiments.

Fig. 5 — Physiological concentration of K⁺ blocks Ca²⁺-dependent DNA degradation in nuclei. (a) TUNEL assay, Nuclei (1 × 10⁴) were incubated under “apoptotic” (40 mM) and “physiological” concentration (140 mM) of K⁺ with 1 mM CaCl₂. Right, fluorescent TUNEL assay. Left, counter staining with DAP I. The data shown represents at least three experiments. (b) Analysis of Ca²⁺-dependent DNA degradation under various concentration of K⁺. DNA was extracted and run on agarose gel electrophoresis. Nuclei (2 × 10⁶) were incubated with 1 mM CaCl₂ together with an increasing concentration of KCl. For each sample, 1 μg of DNA was analyzed. “Ø” denotes control nuclei maintained on ice. The data shown represents one of three independent experiments.