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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Jan 24;1793(3):584–591. doi: 10.1016/j.bbamcr.2009.01.010

Figure 1.

Figure 1

PLTP is present in nuclear protein extracts of SK-N-SH neuroblastoma and human cortical neurons, HCN2 cells, and its nuclear export is chromosome region maintenance 1 (CRM1) dependent. A) Isolated SK-N-SH nuclear proteins from three different samples were resolved using SDS-PAGE, and visualized using Western blot analysis with monoclonal anti-PLTP antibody. B) PLTP Western blot analysis of nuclear proteins isolated from HCN2 cells incubated without (−) or with (+) 10 ng/ml LMB, a specific inhibitor of nuclear export mediated by CRM1, for 24 hours under serum-free conditions. PLTP was identified using monoclonal anti-PLTP antibody. Loading control was evaluated using anti-TATA binding protein (TBP). Secondary antibody controls were negative (not shown).