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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: J Neuroendocrinol. 2009 Jan;21(1):77–81. doi: 10.1111/j.1365-2826.2008.01801.x

Figure 1.

Figure 1

Progesterone (P4) decreases the expression of ERα and ERβ mRNA in a concentration- and time-dependent manner. Representative agarose gels of RT-PCR products qualitatively show relative changes in mRNA levels of ERα and ERβ induced by 24 h exposure to 0-100 ng/mL (∼0-300 nM) P4 (a) and 0-24 h exposure to 10 ng/ml (∼30 nM) P4 (d); β-actin was used as an internal control. The relative levels of ERα and ERβ mRNA after treatment with various P4 concentrations (b, c; n=5) and for various time points (e, f; n=3) were determined quantitatively using real-time PCR. Data show mean (±SEM) expression levels, relative to vehicle-treated controls, as determined by Ct values (cycle number at which the logarithmic fluorescence crosses the threshold) of ERα and ERβ normalised with corresponding Ct values of β-actin. * Denotes p ≤ 0.01 relative to corresponding vehicle-treated control group. The primer sets used were: ERα- F : 5 ′-CATCGATAAGAACCGGAG-3 ′ a n d R : 5 ′-AAGGTTGGCAGCTCTCAT-3′; ERβ - F: 5′-AAAGTAGCCGGAAGCTGA-3′ and R: 5′-CTCCAGCAGCAGGTCATA-3′; β-actin — F: 5′-AGCCATGTACGTAGCCATCC-3′ and R: 5′-CTCTCAGCTGTGGTGGTGAA-3′.