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. Author manuscript; available in PMC: 2009 Nov 15.
Published in final edited form as: J Immunol. 2008 Nov 15;181(10):6995–7001. doi: 10.4049/jimmunol.181.10.6995

Figure 6. Dyn2 does not affect granule polarization.

Figure 6

A, Primary human NK cells were transfected with control siRNA or siRNA directed against Dyn2. After 72 hours, these cells were fixed and stained with anti-Dyn2 (green) to analyze suppression and anti-perforin (red) to label lytic granules. B, Primary human NK cells were tranfected as in A and then conjugated to CMAC-stained 721 target B cells (blue) and incubated for 30 minutes at 37°C. These NK-721 conjugates were then fixed and stained with anti-Dyn2 (green) and anti-perforin (red). C, Conjugates from B were scored for perforin-containing granule polarization to the NK-721 cytolytic synapse. Data shown are representative or 2 experiments performed in triplicate.