Figure 1.

State-based screening using lysate microarrays. Cells are grown in 96-well microtiter plates, treated with small molecules (one compound per well), stimulated with a growth factor, and lysed. The resulting lysates are arrayed onto glass-supported nitrocellulose pads and the arrays are assembled into a microtiter-plate format. Each array is probed with a different pan- or phosphorylation-specific antibody, yielding a quantitative snapshot of the state of the signaling network induced by each small molecule.