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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Nat Methods. 2006 Oct;3(10):825–831. doi: 10.1038/NMETH931

Figure 2.

Figure 2

Evaluation of antibodies for use in lysate microarrays. (a) Antibody to pY1173-EGFR shows no significant cross-reactivity on lysate microarrays. (b) Antibody to pT308-Akt antibody shows only nonspecific signals on lysate microarrays. (c) Antibody to EGFR shows some cross-reactivity on lysate microarrays, resulting in compressed signal ratios relative to the immunoblots. Cellular lysates were analyzed at one concentration on immunoblots (top) and at seven different concentrations on lysate microarrays (twofold dilution series; middle). Lysates in a and b were derived from A431 cells stimulated for different durations with 200 ng/ml of EGF; lysates in c were derived from six cancer cell lines. Quantitative data from the immunoblots and the lysate microarrays were normalized relative to their respective mean values and compared (bottom).

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