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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Nat Methods. 2006 Oct;3(10):825–831. doi: 10.1038/NMETH931

Figure 3.

Figure 3

Lysate microarrays resulting from a state-based screen of 84 kinase and phosphatase inhibitors. (a) A 2.5 × 7.5 cm glass slide with 16 identical lysate microarrays probed with eight different antibodies. Arrays probed with antibodies to pY1173-EGFR and to pT202/pY204-Erk are highlighted. (b) Enlarged views of microarrays shown in a. Top, array probed with antibody to pY1173-EGFR; bottom, array probed with antibody to pT202/pY204-Erk. Locations of lysates derived from cells treated with U-0126 and AG-1478 are highlighted. The EGFR inhibitor AG-1478 causes a decrease in the EGF-induced phosphorylation of EGFR and Erk, whereas the MEK inhibitor U-0126 causes a decrease in phosphorylation of Erk but does not affect that of EGFR. The layout of each microarray is shown in bottom right.

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