Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2010 Aug 1.

Published in final edited form as: Biochem Pharmacol. 2009 Apr 14;78(3):241–248. doi: 10.1016/j.bcp.2009.04.005

Fig. 3 — A, KB-3 cells were transiently transfected for 48 h with GFP and either myc-Bax-pCMV, myc-pCMV, HA-pCMV, HA-Bcl-xL-pCMV or HA-Bcl-xL-pCMV plus myc-Bax-pCMV, as indicated. Fluorescent images (upper panel) were taken at 488 nm. Phase contrast images (at 5x magnification compared to GFP images) are shown in the lower panel. All images were taken with a Zeiss Axiovert S100 TV microscope. B, KB-3 cells were transiently transfected for 48 h with myc-Bax-pCMV, myc-pCMV, HA-pCMV, HA-Bcl-xL-pCMV, or HA-Bcl-xL-pCMV plus myc-Bax-pCMV, and whole cell extracts were prepared and subjected to immunoblotting for Bcl-xL and Bax. GAPDH was used as a loading control. C, KB-3 cells were transiently transfected with myc-Bax-pCMV only or myc-Bax-pCMV plus HA-Bcl-xL-pCMV for 48 h. The relative extent of apoptosis was assessed quantitatively, as described under “Materials and methods.” Apoptosis induced by control plasmids myc-pCMV and HA-pCMV was averaged and taken as a background and subtracted to obtain the data shown. The results represent mean ± S.D. (n = 6) and are representative of two independent experiments. D, KB-3 cells were transiently transfected with control plasmids (left column) or myc-Bax-pCMV plus HA-Bcl-xL-pCMV or myc-Bax-pCMV only for 48 h, and the adherent and non-adherent cells from each transfection condition shown were collected separately, counted, and expressed as percentage of the total number of cells. Data represent the averages of six independent determinations. The means and standard error for each value, expressed as a percentages, are as follows: control plasmids, adherent 60.0 ± 4 %, non-adherent, 40 ± 6 %; Bcl-xL/Bax plasmids, adherent, 36 ± 4 %, non-adherent, 64 ± 1 %; Bax plasmid, adherent, 19 ± 3 %, non-adherent, 81 ± 6 %.

Fig. 3 — A, KB-3 cells were transiently transfected for 48 h with GFP and either myc-Bax-pCMV, myc-pCMV, HA-pCMV, HA-Bcl-xL-pCMV or HA-Bcl-xL-pCMV plus myc-Bax-pCMV, as indicated. Fluorescent images (upper panel) were taken at 488 nm. Phase contrast images (at 5x magnification compared to GFP images) are shown in the lower panel. All images were taken with a Zeiss Axiovert S100 TV microscope. B, KB-3 cells were transiently transfected for 48 h with myc-Bax-pCMV, myc-pCMV, HA-pCMV, HA-Bcl-xL-pCMV, or HA-Bcl-xL-pCMV plus myc-Bax-pCMV, and whole cell extracts were prepared and subjected to immunoblotting for Bcl-xL and Bax. GAPDH was used as a loading control. C, KB-3 cells were transiently transfected with myc-Bax-pCMV only or myc-Bax-pCMV plus HA-Bcl-xL-pCMV for 48 h. The relative extent of apoptosis was assessed quantitatively, as described under “Materials and methods.” Apoptosis induced by control plasmids myc-pCMV and HA-pCMV was averaged and taken as a background and subtracted to obtain the data shown. The results represent mean ± S.D. (n = 6) and are representative of two independent experiments. D, KB-3 cells were transiently transfected with control plasmids (left column) or myc-Bax-pCMV plus HA-Bcl-xL-pCMV or myc-Bax-pCMV only for 48 h, and the adherent and non-adherent cells from each transfection condition shown were collected separately, counted, and expressed as percentage of the total number of cells. Data represent the averages of six independent determinations. The means and standard error for each value, expressed as a percentages, are as follows: control plasmids, adherent 60.0 ± 4 %, non-adherent, 40 ± 6 %; Bcl-xL/Bax plasmids, adherent, 36 ± 4 %, non-adherent, 64 ± 1 %; Bax plasmid, adherent, 19 ± 3 %, non-adherent, 81 ± 6 %.