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Nuclear distribution in juvenile (A) and adult (B) anaerobic light fibers. Cross sections of fixed light fibers from WGA-injected animals, which were treated with 4′,6-diamidino-2-phenylindole, a blue-fluorescent probe for nuclei. Green-fluorescent WGA staining identifies fiber sarcolemma. In the small fibers of the juvenile, nuclei are found almost exclusively at the fiber edge; in the large fibers of the adult, there is a high density of SS and IM nuclei.
