Fig. 1.

Cytological characterization of purified murine alveolar type II (AT2) cells. A: purities of freshly isolated AT2 cells from SvEv mice assessed by immunostaining for pro-surfactant protein C (SPC; green). Cytospin preparations reflect purities of 91 ± 1% AT2 cells. Nuclei were counterstained with propidium iodide (red). Results are representative of 24 independent experiments. B: immunoreactivity of claudin-18 (green) in mouse alveolar epithelial cell monolayers (MAECM) grown on a laminin 5-coated polycarbonate filter for 6 days. Nuclei were counterstained with propidium iodide (red). Scale bar, 50 μm. Original magnification ×40.