Fig. 5.

CD45⁺ and procollagen-1⁺ cells were detected in the proximal vascular wall tissue. Immunohistochemistry using anti-CD45 antibody shows the accumulation of CD45⁺ cells (arrows in A). Immunofluorescent staining using procollagen-1 antibody identified clusters of procollagen-1⁺ cells. Nuclei were counterstained with DAPI (blue). The images on the right are magnified sections of the main panels highlighting either CD45⁺ (A) or procollagen⁺ cells (B). Scale bars reflect 100 μm in A and 25 μm in B.