Fig. 2.
Effect of LPS on mRNA expression for gVPLA2. mRNA expression for gVPLA2 caused by LPS in wild-type (WT) littermate control (pla2g5+/+) or gVPLA2 knockout (pla2g5−/−) mice was measured in lung homogenates by RT-PCR as described in materials and methods. The upregulation of mRNA was normalized as the ratio of mRNA/18S compared with vehicle-stimulated control airways. RT-PCRs were performed in triplicate. The data represent the means ± SE of 6 samples; *P < 0.05 and **P < 0.001 by Student's t-test.