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. 2009 Mar 27;296(6):L1019–L1030. doi: 10.1152/ajplung.90596.2008

Fig. 3.

Fig. 3.

Effect of TTF-1 and CEACAM6 knockdown. Freshly isolated epithelial cells were electroporated with small inhibitory RNA (siRNA) oligonucleotide (si) or scrambled control oligonucleotide (si-ct) and then cultured for 72 h. A: Western blot in representative experiment of TTF-1 knockdown. DCI alone (lane 3) increased TTF-1, CEACAM6, and SP-B compared with control (lane 4, Waymouth medium). Addition of TTF-1 siRNA oligos in the presence of DCI (lane 1) reduced levels of TTF-1, CEACAM6, and SP-B compared with the scrambled control oligo (lane 2). B: densitometric data comparing knockdown of TTF-1 vs. knockdown of CEACAM6. Knockdown of TTF-1 (filled bars) significantly reduced content of TTF-1 (46 ± 6% of control), CEACAM6 (20 ± 2% of control), and SP-B (26 ± 5% of control) in 4 experiments (all *P < 0.01). In contrast, knockdown of CEACAM6 (n = 3, gray bars) reduced content of CEACAM6 (12 ± 3% of control, *P < 0.01) but did not affect either TTF-1 or SP-B.