Fig. 3.

The specificity of isolectin B4 binding and DiI-Ac-LdL uptake as markers of ECs. A: the binding of FITC-labeled isolectin B4 to various murine cells was assessed by FACS analysis. Rat IgG, blue tracing; isolectin B4, red tracing. Isolectin B4 bound not only to ECs but to the murine 3T3 fibroblast and B16 melanoma lines as well. B: the uptake of DiI-Ac-LdL by murine ECs was determined by immunofluorescence microscopy. 3T3 murine fibroblasts demonstrated the capacity to take up DiI-Ac-LdL, although less than that of the ECs.