Fig. 4.

Purification of ECs from mouse lung. A: the major steps of the protocol used in this paper for the isolation of ECs from murine lung. B: shown are cells from digested lung that had been plated and cultured for 24 h and then stained immunofluorescently with an anti-PECAM-1 antibody. The staining demonstrates the presence of clusters and aggregations of ECs. Cell nuclei are detected by DAPI staining in blue. C: FACS, using ICAM-2 antibody, of cells from digested lung that had been cultured for 2–3 days. Background, black tracing; ICAM-2, red tracing. A population of ICAM-2-expressing (endothelial) cells was present (circle) and was selected for sorting.