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. Author manuscript; available in PMC: 2010 Jun 30.
Published in final edited form as: Neuroscience. 2009 Mar 31;161(2):403–412. doi: 10.1016/j.neuroscience.2009.03.063

Figure 1.

Figure 1

SPTB Numb-induced neurite outgrowth requires Ca2+ influx and MAP kinase activation. A. Phase-contrast images of cells expressing SPTB/SPRR Numb that had been incubated for five days in the presence of 0.2% dimethylsulfoxide, 10 μM nifedipine (Nif), 0.5 μM ω-contoxin (CgTx), 10 μM genistein, 5 μM PD98095, 0.5 mM EGTA or in the absence of any treatment (untreated control) and image of cells expressing vector. Drugs were added into the culture medium (DMEM without serum) of 35 mm Petri dishes 3–4 hrs after cells were plated into the dishes. B. Quantification of neurite length (B1) and the percentage of cells with neuritis (B2) in SPTB/SPRR cells that had been exposed for five days to the indicated treatments. Values are the mean ± SEM of the measurements made in four cultures in different days per treatment condition (100–150 cells were evaluated per treatment condition). *p<0.05, **p<0.01, ***p<0.001 compared to either no-treated or DMSO treated group in SPTB/SPRR Numb, ANOVA followed by Holm-Sidak methods for multiple comparison. Horizontal bar: 10 μm.