Table.
Real Time polymerase chain reaction (PCR) measurements of K+-channel subunits in the mature and immature ductus arteriosus of sheep, baboons and mice. ΔCT(MDH-gene) represents the difference in cycle threshold (CT) between the expression of the housekeeping gene Malate dehydrogenase (MDH) and the gene of interest. Each unit of ΔCT(MDH-gene) represents a 2-fold increase in a gene's mRNA. The more negative the ΔCT(MDH-gene), the fewer the number of starting copies of a gene (mRNA). Number of separate animals used: sheep (immature=7, mature=7); baboon (immature=10, mature=10); mouse litters (immature=10, mature=9). From each litter of mice we obtained between 10-12 ductus. ↑ p<0.05, mature significantly greater than immature; ↓ p<0.05, mature significantly less than immature; - p>0.05, mature not significantly different from immature.
| Gene | gestation | Sheep | Baboon | Mouse | |||
|---|---|---|---|---|---|---|---|
| ΔCT(MDH-gene) | ΔCT(MDH-gene) | ΔCT(MDH-gene) | |||||
| mean | (sd) | mean | (sd) | mean | (sd) | ||
| Kv1.2 | immature | -8.0 | (0.8) | -3.3 | (1.3) | -3.9 | (0.4) |
| Mature | -5.6 | (0.9) | -1.1 | (0.9) | -4.8 | (0.4) | |
| p<0.05 | ↑ | ↑ | ↓ | ||||
| Kv1.5 | immature | -7.6 | (1.5) | 1.2 | (0.2) | -3.6 | (0.2) |
| Mature | -4.5 | (0.5) | 0.7 | (0.2) | -3.2 | (0.3) | |
| p<0.05 | ↑ | ↓ | ↑ | ||||
| Kv2.1 | immature | -4.0 | (0.6) | -3.8 | (0.8) | -4.7 | (0.3) |
| Mature | -3.0 | (0.2) | -3.0 | (0.5) | -3.6 | (0.8) | |
| p<0.05 | ↑ | ↑ | ↑ | ||||
| Kv9.3 | immature | -6.6 | (1.4) | -6.5 | (1.6) | -1.5 | (0.3) |
| Mature | -4.2 | (0.9) | -4.7 | (1.0) | -1.6 | (0.4) | |
| p<0.05 | ↑ | ↑ | - | ||||
| Kvβ1.2 | immature | -11 | (0.5) | -5.1 | (1.2) | -3.9 | (0.3) |
| Mature | -11 | (1.0) | -3.3 | (0.8) | -3.9 | (0.6) | |
| p<0.05 | - | ↑ | - | ||||
| Kvβ1.3 | immature | -2.6 | (0.5) | -3.0 | (1.2) | -1.5 | (0.2) |
| Mature | -1.3 | (0.3) | -1.2 | (0.8) | -2.0 | (0.3) | |
| p<0.05 | ↑ | ↑ | ↓ | ||||
| KIR6.1 | immature | -6.3 | (0.3) | -1.7 | (1.0) | -4.3 | (0.4) |
| Mature | -5.7 | (0.3) | -2.0 | (1.0) | -2.4 | (0.7) | |
| p<0.05 | ↑ | - | ↑ | ||||
| SUR2 | immature | -4.0 | (0.2) | -0.8 | (0.2) | -6.5 | (0.5) |
| Mature | -3.3 | (0.3) | -1.2 | (0.2) | -3.2 | (0.4) | |
| p<0.05 | ↑ | ↓ | ↑ | ||||
| BKCa | immature | -9.0 | (0.3) | -1.8 | (0.3) | -10.6 | (0.6) |
| mature | -8.6 | (0.4) | -1.8 | (0.3) | -9.5 | (0.9) | |
| p<0.05 | - | - | - | ||||
| BKcaβ1 | immature | -2.6 | (0.3) | -0.5 | (0.3) | -4.8 | (0.6) |
| mature | -1.2 | (0.4) | 0.1 | (0.3) | -3.7 | (0.9) | |
| p<0.05 | ↑ | ↑ | ↑ | ||||