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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Cell Microbiol. 2008 Aug 5;10(11):2257–2270. doi: 10.1111/j.1462-5822.2008.01205.x

Fig. 4. The absence of substantial ROS production in PMNs exposed to liquid-grown, exponential-phase Gc requires live bacteria capable of de novo protein synthesis.

Fig. 4

A. Liquid-grown, heat-killed Opa- Gc stimulate ROS production in PMNs. PMNs were left uninfected or were infected with liquid-grown Opa- MS11 Gc that were live (MOI 120) or killed by heating at 56 °C for 30 min (HK; concentration equivalent to MOI 250). ROS production was measured as in Fig. 1A.B. Viable Opa- Gc treated with antibiotics that inhibit protein synthesis stimulate PMN ROS production. PMNs were infected with liquid-grown Opa- FA1090 Gc at a MOI of 100. Gc were exponentially growing (live), heat-killed (HK), or treated with sublethal concentrations of the protein synthesis inhibitors chloramphenicol (Cm) or tetracycline (Tet). Uninfected PMNs did not generate any detectable ROS (data not shown).