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Expression of tTA is confined to the female fat body and is inhibited by tetracycline. Climbing third instar larvae were sexed, dissected, and stained for β-galactosidase expression (25). Strong staining was seen in fat body of female larvae raised on normal medium (A) but not medium that contained tetracycline (10 μg/ml; B). Little staining above background level was seen in fat body from male larvae raised on either normal medium (C) or medium supplemented with tetracycline (D).
