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. Author manuscript; available in PMC: 2009 Jun 8.

Published in final edited form as: Mol Cell Neurosci. 2008 Mar 4;38(1):110–116. doi: 10.1016/j.mcn.2008.02.005

(A) Rat PND5-8 CGNs were treated with or without BDNF and one of the following calcium flux inhibitors: 2-APB, APV or nimodipine. Only the IP₃-channel inhibitor, 2-APB, inhibited the BDNF-induced CREB phosphorylation. (B) CGNs were primed with BDNF in the presence or absence of these inhibitors and grown on MAG-expressing (grey bars) or control (black bars) CHO cells. The only inhibitor that blocked the BDNF-induced priming effect was the IP₃-channel inhibitor, 2-APB. Histograms show the mean length (as a percent of control) for the longest neurite per neuron (±SEM) for 175-200 neurons and represent a composite of 3 separate experiments.

(A) Rat PND5-8 CGNs were treated with or without BDNF and one of the following calcium flux inhibitors: 2-APB, APV or nimodipine. Only the IP₃-channel inhibitor, 2-APB, inhibited the BDNF-induced CREB phosphorylation. (B) CGNs were primed with BDNF in the presence or absence of these inhibitors and grown on MAG-expressing (grey bars) or control (black bars) CHO cells. The only inhibitor that blocked the BDNF-induced priming effect was the IP₃-channel inhibitor, 2-APB. Histograms show the mean length (as a percent of control) for the longest neurite per neuron (±SEM) for 175-200 neurons and represent a composite of 3 separate experiments.