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. Author manuscript; available in PMC: 2010 Feb 7.
Published in final edited form as: J Mol Biol. 2008 Nov 14;385(5):1481–1497. doi: 10.1016/j.jmb.2008.10.097

Figure 5.

Figure 5

Strips extracted from various solid-state 3D triple resonance spectra are shown. The assignment of the fragment, L137 to L131 using strips from 3D NCACX, NCACB and NCOCX spectra and the analysis of sequential cross peaks derived from 1,3-αB and 2-αB samples are illustrated. The labeling scheme, experiment type, and the mixing time are indicated on the right hand side. The experiment type is indicated with NCOCX, NCACX or NCACB with the prefixes U, 1,3G, and 2G, indicating spectra acquired on U-, 1,3-, and 2-αB samples, respectively. In NCACX spectra, the 15N and Cα resonances of residue i are correlated with the side chain of residue i. In NCACB, the 15N and Cα resonances of residue i are correlated with the Cβ of residue i. NCOCX spectra provide the corresponding sequential information and correlate 15N of residue i and C′ of residue i-1 with the side chain of residue i-1. (See main text for details).