(A) Emission spectra of ANS in the presence of apoE3 (a), apoE3 (1–272) (b), apoE3 (1–260) (c), apoE3 22-kDa (d), and buffer (e). (B) ANS fluorescence intensity ratio of the C-terminal-truncated mutants to full-length apoE. The results from at least 2 independent experiments, each done in triplicate, are shown.