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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Neurobiol Aging. 2006 Dec 13;29(3):319–328. doi: 10.1016/j.neurobiolaging.2006.10.027

Figure 3.

Figure 3

Increase in trisomy 21 aneuploidy in human cells induced by transfected normal and FAD mutant PS-1. (A) Examples of FISH with the LSI TEL/AML1 ES dual color probe used to detect both chromosome 12 (Spectrum Green) and 21 (Spectrum Orange) in PS-1 transfected hTERT cells. DAPI was used as a counter-stain. Chromosome 21 trisomy (right) reveals aneuploidy in a PS-1 transfected hTERT cell which is normal (disomic) for chromosome 12. A typical non-transfected hTERT cell (left) reveals normal (disomy) karyotype as evidenced by 2 red signals and 2 green signals. (B) Quantitative FISH of hTERT cells transfected with WT or mutant (M146L) PS-1 plasmids revealed significantly higher aneuploidy (trisomy 21) in both when compared to cells transfected with the empty vector.