Skip to main content
. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: J Cell Physiol. 2009 Mar;218(3):522–536. doi: 10.1002/jcp.21624

Fig. 6.

Fig. 6

Redistribution of CTB-bound GM1 was not correlated with full acrosomal exocytosis. Dual labeling experiments using CTB (AlexaFluor 555-conjugate) and PNA (AlexaFluor 488-conjugate) were performed in unfixed cells. (A) Two dead cells showing CTB redistributed to the PAPM region and no PNA labeling indicating intact acrosomal vesicles. (B) A control live cell with an intact acrosomal vesicle. CTB is in the APM and there is no PNA labeling. (C) A dead sperm cell with acrosomal membranes exposed in the region of the AA had a diffuse CTB labeling with increased intensity in the AA and SAR/PAPM. PNA labeled the AA, revealing a partial acrosomal exocytosis. (D) A damaged cell in which parts of the APM and the OAM were lost, had a loss of CTB labeling and exposure of the acrosomal membranes in the ES. All panels show representative and consistent data obtained from at least 4 separate trials, with multiple cells (>100) observed per trial.