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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Brain Behav Immun. 2008 Sep 12;23(3):309–317. doi: 10.1016/j.bbi.2008.09.002

Fig.5. Higher percentage of IL-1β+ and IL-10 positive microglia in aged mice compared to adults following peripheral LPS injection.

Fig.5

Adult (3-6 m) and aged (18-20 m) mice were challenged with saline or LPS i.p. and microglia were isolated 4 h later by Percoll density gradient. Cells were subjected to the BD Cytofix/Cytoperm Plus fixation/permeabilization protocol as described in the Methods. Representative bivariate dot plots of Percoll isolated cells stained with A) anti-CD11b-APC and anti-IL-1β-FITC or B) anti-CD11b-APC and anti-IL-10-FITC. Average percentage of cells that were C) CD11b+/IL-1β+ and D) CD11b+/IL-10+. Bars represent the mean ± SEM (n=3-6).