Table 1.
Summary of ATPase activity and motility of myosin reconstituted with various mutants of LC1
| Myosina (S1) | Vmax (s−1) | Km (μM) | n | Vactin (μm/s) |
|---|---|---|---|---|
| WT | 7.7 ± 1.3 | 1.7 ± 1.0 | 4 | 5.08 ± 0.89 |
| Δ13 | 13.3 ± 1.8 | 10.4 ± 4.7 | 8 | 6.39 ± 0.80 |
| Δ29 | 15.9 ± 6.2 | 21.7 ± 10.7 | 3 | 7.61 ± 1.35 |
| Δ43 | 15.1 ± 0.8 | 18.2 ± 3.0 | 7 | 7.68 ± 1.07 |
| A1+A2 | ---- | ---- | 8.09 ± 1.12 |
a
Myosin reconstituted with light chains was used only for the in vitro motility assays. The kinetic assays were all performed with S1(A2) exchanged with the various light chains cited here, as described in Methods. The mean and standard deviation for n independent determinations of ATPase activity are shown. The motility values (mean ± SD) represent two independent determinations of 30–40 filaments each.