Table 2.
Sample | Donor/Acceptor | E (%)a | R0 (Ǻ)b | R (Å)c |
---|---|---|---|---|
1 | DAN/DDPM | 11 9.2 |
29 | 41 42 |
2 | DAN/DABM | 24 28 |
36 | 44 42 |
3 | DAN/IAF | 69 61 |
42 | 37 39 |
Efficiency of energy transfer between donor/acceptor probes on the cysteine residues in the -C3/C45 mutant (Figure 1).
Forster distance for which the efficiency of transfer is 50% (values taken from the literature).
Distance between the various donor/acceptor probes. DAN is the donor dansyl moiety of 1,5-IAEDANS; see chemical name for acceptor dyes under abbreviations.