Table 3.

Immunoglobulin gene usage for variable heavy chains of human anti-Env mAbs

mAb	Cells	IGHV	IGHD	D-RF	IGHJ	Reference
Anti- CD4bd mAbs
b12a	Phage	1-3*01	2-21	2	6	(Barbas et al., 1993)
GP44	EBV	1-46*01	3-16	3	4	(van der Donk et al 1994)
GP68	“	1-69*01	3-16	2	4	(van der Donk et al 1994)
S1-1	Hybridoma	1-69*09	2-2	3	4	(Moran et al., 1993)
21h	EBV	3-11*03	4-17	3	5	(Bagley et al., 1994)
b3a	“	3-15*06	3-9	2	6	(Barbas et al., 1993)
b13a	“	3-30*04	3-9	2	4	(Barbas et al., 1993)
m14	“	4-30-4*01	3-10	1	4	(Zhang et al., 2004)
15e	“	4-39*02	2-8	1	6	(Bagley et al., 1994)
F105	Hybridoma	4-59*01	4-17	2	5	(Marasco et al., 1992)
GP13	EBV	5-a*03	3-9	2	6	(van der Donk et al 1994)
Anti-CD4i mAbs
411gb	EBV	1-24*01	1-26	1	4	(Huang et al., 2004)
X5	Phage	1-69*01	3-22	2	4	(Huang et al., 2004)
E51c	EBV	1-69*01	2-2	3	6	(Huang et al., 2004)
412dc	“	1-69*01	4-4	2	2	(Huang et al., 2004)
47e3	“	1-69*01	4-17	2	6	(Huang et al., 2004)
23eb	“	1-69*06	3-3	2	6	(Huang et al., 2004)
17b6	“	1-69	nd	nd	1	(Thali et al., 1992)
Sb1d,f	Phage	1-69	nd	nd	6	(Huang et al., 2004)
C12d,f	“	1-69	nd	nd	4	(Huang et al., 2004)
m16f	“	1-69	nd	nd	6	(Huang et al., 2004)
16c	“	1-f*01	5-12	3	4	(Huang et al., 2004)
48d	EBV	1-f*01	1-26	2	3	(Thali et al., 1992)
Anti-gp41 mAbs
DaB2B3	EBV	1-46*01	3-16	3	3	(David et al., 1995b)
Ab 31	Phage	1-46*01	2-2	1	3	(de Haard et al., 1998)
246-D+	Hybridoma	1-69*01	6-19	2	4	(Xu et al., 1991)
181-D+	“	1-69*01	3-16	1	6	(Xu et al., 1991)
50-69D+	“	1-69*01	2-21	2	4	(Gorny et al., 1989)
No.86	“	1-69*06	3-9	2	4	(Moran et al., 1993)
1281+	“	1-69*06	3-9	1	5	(Gorny et al., 2000)
126-7+,e,h	“	1-69*06	2-2	3	4	(Xu et al., 1991)
167-7+	“	1-69*09	3-10	3	4	(Xu et al., 1991)
240-D+	“	1-69*10	3-9	2	5	(Xu et al., 1991)
4E10f	“	1-69	3-16	nd	1	(Kunert et al., 2004)
F240	“	3-11*01	3-22	2	5	(Cavacini et al., 1998)
1379+	“	3-11*03	4-17	1	4	(Gorny et al., 2000)
NG3B7	EBV	3-23*04	1-26	3	3	(David et al., 1995b)
1342+	Hybridoma	3-23*04	3-10	3	4	(Gorny et al., 2000)
3D6	“	3-9*01	3-22	2	3	(Felgenhauer et al. 1990)
120-16e	EBV	4-30-2*01	5-12	3	2	(Andris et al., 1991)
1367+	Hybridoma	4-30-4*01	2-2	2	6	(Gorny et al., 2000)
Z13	Phage	4-59*03	3-16	3	6	(Zwick et al., 2001)
98-6e	EBV	4-61*02	4-23	3	4	(Andris et al., 1991)
2F5g	Hybridoma	2-5*10	nd	nd	6	(Kunert et al., 1998)

^a,b,c,d,eThese mAbs were derived from five HIV-infected individuals;

^fonly VH nucleotide sequence available;

^gnucleotide sequence is not available;

^hmAb 126-7 was previously known as 126-6;

⁺the mAbs sequenced in our laboratory for this study; nd – not defined. D-RF, D gene reading frame.