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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Int J Parasitol. 2008 Dec 10;39(6):635–644. doi: 10.1016/j.ijpara.2008.10.015

Fig. 1.

Fig. 1

Reverse transcriptase-PCR analysis of RNA editing of several mitochondrial transcripts in promastigotes and axenic amastigotes of Leishmania donovani. (A) Amplification of mRNA derived from pan-edited cryptogenes ND8 (G1), ND9 (G2), G3, G4, ND3 (G5) and RPS12 (G6) using RNA isolated from purified kinetoplast-mitochondria of promastigotes, K. Control amplifications of the respective gene regions, D, were used to identify pre-edited cDNA products. Arrows indicate bands with the sizes expected for fully edited products that were used for cloning and sequencing. A 1 kb DNA ladder (Invitrogen) was used as a size marker, M. The amplified products were resolved in 3% NuSieve agarose gels. (B) Amplification of mRNA derived from 5′-edited and internally edited mRNA encoded by the genes ND7, COIII, Cyb and A6 using total cell RNA isolated from promastigotes (P) and axenically grown amastigotes (A).