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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Int J Parasitol. 2008 Dec 10;39(6):635–644. doi: 10.1016/j.ijpara.2008.10.015

Fig. 4.

Fig. 4

Protein levels of mitochondrial proteins in different trypanosomatids. (A) Mitochondrial extracts from Leishmania donovani amastigote (LdA), L. donovani promastigote (LdP), Leishmania tarentolae (Lt) and Trypanosoma brucei (Tb) cells were analyzed by Western blot. The antisera used are shown to the right of each panel. (B) Mitochondrial extracts and whole cell lysates were analyzed by Western blotting with antibodies against Mitochondrial RNA-binding proteins 1 and 2 (MRP1/2) and adenylate kinase 2 (AK2). In addition, mitochondrial extract were probed for MRP-AP1, MRP-AP2 and Rieske proteins. (C) Differentiation of L. donovani was verified by probing cell lysates for an amastigote-specific marker A2. Notice that this protein is represented by multiple bands (Charest and Matlashewski, 1994). Rieske and p18 proteins were probed as indicated. Total cell lysate loading was normalized by using the same number of cells (70 × 106) per lane and loading of mitochondrial lysates was normalized by using 50 μg protein per lane.