Table 2.
Chromatin compaction assessment by sedimentation coefficients of 12-unit oligonucleosomes at four different conditions with regular tails, selectively neutralized tails, and all tails neutralized (experimental values are provided in square brackets)
| Array type | S20,w, ΔS20,w (S) a | |||
|---|---|---|---|---|
| loMSb | hiMSc | MS-LHd | MS-LH-Mge | |
| regular | 30.0 ± 0.2 [29.8f] | 38.5 ± 1.2 [37.5f] | 48.8 ± 1.7 [55.6g] | 53.2 ± 2.5 [60g] |
| all tails neutral | 25.8 ± 0.2 (-4.2) [25h] | 31.2 ± 0.2 (-7.3) [32h] | 44.9 ± 1.3 (-3.9) | 48.7 ± 1.9 (-4.5) |
| H3 neutral | 25.8 ± 0.2 (-4.2) | 33.2 ± 0.3 (-5.3) | 45.6 ± 1.2 (-3.2) | 49.5 ± 2.0 (-3.7) |
| H4 neutral | 29.7 ± 0.2 (-0.3) | 34.9 ± 0.5 (-3.6) | 46.2 ± 1.5 (-2.6) | 49.4 ± 1.9 (-3.8) |
| H2A neutral | 29.6 ± 0.2 (-0.4) | 36.3 ± 0.8 (-2.2) | 48.5 ± 1.5 (-0.3) | 52.1 ± 2.2 (-1.1) |
| H2B neutral | 29.6 ± 0.2 (-0.4) | 35.1 ± 0.6 (-3.4) | 48.0 ± 1.4 (-0.8) | 52.3 ± 2.3 (-0.9) |
a
ΔS20,w shown in parenthesis is computed as the change in the sedimentation coefficient of tail neutralized oligonucleosomes relative to regular oligonucleosomes under same salt/linker histone conditions
b
loMS = 0.01 M Na+
c
hiMS = 0.15 M Na+
d
MS-LH = 0.15 M Na+, with linker histones
e
MS-LH-Mg = 0.15 M Na+, with linker histones and Mg2+
g
Experimental values from Grigoryev et al57
h
Experimental values from Fletcher et al79