Table 2. Mutations in the Qn domain and their effects on IcmQ.

Mutations	I13N	P25L, S29F	Δ10-12	Δ40-42
Stable IcmQ mutant protein in Legionella pneumophila	Yes	Yes	Yes	Yes
Intracellular growth in macrophages	+/-	No	Yes	No
DotA secretion	No	No	No	No
IcmR-IcmQ complexes†	nd	Yes	nd	Yes
Vesicle association of the IcmQ mutant protein	nd	Yes	nd	Yes
Calcein dye release by the IcmQ mutant protein*	nd	Yes (75%)	nd	+/- (23%)

nd- not determined

Data for IcmQ stability in Legionella pneumophila, DotA secretion and intracellular growth in mouse macrophages are shown in Figure 7.

^†Complex formation was measured with recombinant proteins and is based on their mobility on a Superose-12 HR column and crosslinking with DTSSP.

^*Wild type IcmQ released ∼64% of the dye under these conditions.