Table 2.

Summary of secondary imatinib resistant KIT mutations evaluated by D-HPLC and AS–PCR

Patient no.	Treatment	Location	Primary KIT mutations	Secondary KIT mutation frequencies
				DHPLC		AS–PCR*	AS–PCR*
				∼15%		>5%	5–0.5%
1	IM	Serosal nodule (1)	Exon 9	NMF	NMF	NMF	NMF
2	IM, SU	Serosal nodule right lower quadrant	Exon 9	NMF	NMF	NMF	NMF
		Omental nodule	Exon 9	NMF	NMF	NMF	V654A
		Periumbilical tumour	Exon 9	Exon 17	N822Y	NMF	D820G
		Serosal nodule right upper quadrant (1)	Exon 9	Exon 17	N822K	N822K (T → G)	V654A
		Serosal nodule right upper quadrant (2)	Exon 9	Exon 17	N822K	N822K (T → G)	NMF
3	IM, SU	Stomach (1)	Exon 11	Exon 17	D816H	NMF	NMF
4	IM	Liver metastasis	Exon 11	Exon 18	A829P	NMF	NMF
		Stomach (1)	Exon 11	Exon 13	V654A	V654A	NMF
5	IM, SU	Liver metastasis	Exon 11	Exon 13	V654A	V654A	D820G
		Mesenteric nodule (1)	Exon 11	Exon 17	N822Y	NMF	N822K (T → A)
		Mesenteric nodule (2)	Exon 11	Exon 17	N822Y	NMF	N822K (T → G)
		Nodule attached to gallbladder	Exon 11	Exon 17	D820G	D820G	NMF
6	IM	Intra-abdominal 1	Exon 11	Exon 13	V654A	V654A	NMF
		Intra-abdominal 2	Exon 11	Exon 13	V654A	V654A	NMF
7	IM, SU	Stomach	Exon 11	NMF	NMF	NMF	NMF
		Liver metastasis	Exon 11	NMF	NMF	NMF	NMF
		Serosal nodule	Exon 11	NMF	NMF	NMF	NMF
8	IM	Abdominal tumour (1)	Exon 11	NMF	NMF	NMF	NMF
		Abdominal tumour (2)	Exon 11	Exon 17	D820Y	NMF	NMF
		Abdominal tumour (3)	Exon 11	Exon 17	D820Y	NMF	NMF
9	IM, SU	Mesenteric nodule 1	Exon 11	Exon 13	V654A	V654A	NMF
		Skin metastasis	Exon 11	Exon 13	V654A	V654A	NMF
		Mesenteric nodule 4	Exon 11	Exon 13	V654A	V654A	N822K (T → A)
10	IM	Stomach wall (3)	Exon 13	NMF	NMF	NMF	D820G
11	IM, SU	Tumour mid-abdomen	Exon 13	NMF	NMF	NMF	NMF
		Serosal nodule left upper quadrant (1)	Exon 13	Exon 14	T670I	NMF	NMF
		Serosal nodule ileum (1)	Exon 13	Exon 17	D816H	NMF	NMF
		Serosal nodule ileum (2)	Exon 13	Exon 17	D816H	NMF	NMF
		Serosal nodule terminal ileum	Exon 13	Exon 17	D816H	NMF	NMF
		Serosal nodule left upper quadrant (2)	Exon 13	Exon 17	D816H	NMF	NMF
12	IM, SU	Omental nodule	NMF	NMF		NMF	NMF
		Liver metastasis (1)
		Tumour sigmoid wall
		Liver metastasis (2)
13, NF Type I	IM, SU	Serosal nodule adjacent to bowel	NMF	NMF		NMF	NMF
		Nodule ligamentum of Tritz
		Nodule duodenum
		Mesenteric nodule
14	IM, SU	Perigastric mass (1)	NMF	NMF		NMF	NMF
		Perigastric mass (2)
		Perigastric mass (3)
		Liver metastasis
		Large nodule lesser curvature (1)
		Large nodule lesser curvature (2)

^*AS–PCR detects: exon 13, V654A, exon 17, D820G, N822K (T → A), N822K (T → G), Y823D.

pt, patient; NMF, no mutation found.