Skip to main content
. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: J Mol Biol. 2007 Aug 22;373(4):990–1005. doi: 10.1016/j.jmb.2007.08.038

Figure 4. Quantitative measurement of the CDK4-inhibitory activities of gankyrin proteins.

Figure 4

The in vitro kinase assays were performed as described in Figure 3B, and the incorporation of 32P into the Rb substrate was quantitated using ImageQuant (Molecular Dynamics). IC50 was defined as the inhibitor concentration at which half of the maximum inhibition can be obtained. The experimental error for this assay was estimated to be ± 30%. All assays were performed in triplicate, and P16 was used as a positive control.