Figure 4.

Tracings of projected confocal z-series of RGC axon arbors expressing β-catenin deletion mutants confirm morphological phenotypes. Compared with controls, RGC axons that express ΔARM have two distinct phenotypes. They either do not arborize (B) or they arborize but have abnormally long and tangled branches (C). NTERM-expressing arbors (D) are similar to the subpopulation of ΔARM arbors with no or few branches (B). CTERM-expressing arbors (E) are similar to the subpopulation of ΔARM arbors with abnormally long, tangled branches (C). For each condition (A-E), two examples of arbors are shown. In each example of an arbor, two (or three) (B, left) tracings are shown that correspond to confocal images taken ∼ 24 hr apart. The first tracing of each example corresponds to a confocal image taken at stage 45/46. The left most arbor tracing in A-E corresponds, respectively, to the actual confocal z-series projection image shown in Figure 3A-E. Stippling over a single arbor branch in C and E illustrates a particularly tangled branch. Rostral is to the top and lateral is to the right in all images. Scale bars, 20 μm (A-E).