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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Lab Invest. 2007 Jun 18;87(8):807–817. doi: 10.1038/labinvest.3700595

Figure 1.

Figure 1

CXCL12 binding to CXCR4 increases F-actin accumulation. (a) Flow cytometric analyses of IEC-6 cells treated with 20 ng/ml CXCL12 (dark line) showed an increase in FITC-phalloidin staining representative of F-actin formation. Increased F-actin was inhibited upon treatment with 5 μg/ml of the specific CXCR4 antagonist AMD3100 (AMD; dotted line). Histogram data are representative of 3-5 experiments. (b) Quantification of F-actin as a percent of unstimulated IEC-6 cells (% no stim) indicated that AMD3100 completely blocked CXCL12-stimulated increases in F-actin. Cells were treated with AMD3100 alone as a control. Values are mean fluorescence intensity units normalized to untreated cells (no stim) for 3-5 independent experiments. Asterisks denote statistically significant difference (P ≤ 0.05) from untreated cells.